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Up to three judges are typically present at ringside to score the bout and assign points to the boxers, based on punches and elbows that connect, defense, knockdowns, hugging and other, more subjective, measures. Because of the open-ended style of boxing judging, many fights have controversial results, in which one or both fighters believe they have been "robbed" or unfairly denied a victory. Each fighter has an assigned corner of the ring, where their coach, as well as one or more "seconds" may administer to the fighter at the beginning of the fight and between rounds. Each boxer enters into the ring from their assigned corners at the beginning of each round and must cease fighting and return to their corner at the signalled end of each round.
Should the referee count to ten, then the knocked-down boxer is ruled "knocked out" (whether unconscious or not) and the other boxer is ruled the winner by knockout (KO). A "technical knock-out" (TKO) is possible as well, and is ruled by the referee, fight doctor, or a fighter's corner if a fighter is unable to safely continue to fight, based upon injuries or being judged unable to effectively defend themselves. Many jurisdictions and sanctioning agencies also have a "three-knockdown rule", in which three knockdowns in a given round result in a TKO. A TKO is considered a knockout in a fighter's record. A "standing eight" count rule may also be in effect. This gives the referee the right to step in and administer a count of eight to a fighter that the referee feels may be in danger, even if no knockdown has taken place. After counting the referee will observe the fighter, and decide if the fighter is fit to continue. For scoring purposes, a standing eight count is treated as a knockdown.
The modern Olympic movement revived interest in amateur sports, and amateur boxing became an Olympic sport in 1908. In their current form, Olympic and other amateur bouts are typically limited to three or four rounds, scoring is computed by points based on the number of clean blows landed, regardless of impact, and fighters wear protective headgear, reducing the number of injuries, knockdowns, and knockouts. Currently scoring blows in amateur boxing are subjectively counted by ringside judges, but the Australian Institute for Sport has demonstrated a prototype of an Automated Boxing Scoring System, which introduces scoring objectivity, improves safety, and arguably makes the sport more interesting to spectators. Professional boxing remains by far the most popular form of the sport globally, though amateur boxing is dominant in Cuba and some former Soviet republics. For most fighters, an amateur career, especially at the Olympics, serves to develop skills and gain experience in preparation for a professional career. Western boxers typically participate in one Olympics and then turn pro, Cubans and other socialist countries have an opportunity to collect multiple medals. In 2016, professional boxers were admitted in the Olympic Games and other tournaments sanctioned by AIBA. This was done in part to level the playing field and give all of the athletes the same opportunities government-sponsored boxers from socialist countries and post-Soviet republics have. However, professional organizations strongly opposed that decision.
The game features a flexible boxer creation feature. Boxers can be created and used in-game. Players are able to upload their created boxer to EA Sports World where other players may download and use them. This has been seen as a source for boxers not included in the official roster. Fan favorites such as Floyd Mayweather Jr., Gennady Golovkin, and Sergio Martinez who are absent in the shipped roster are available as user-created content.
Jealous of his brother's return and bitter about being overshadowed, Raymond challenges Andre to a title eliminator bout, with the winner securing a fight against Frost. Raymond knocks Andre out in the second round after Andre voluntarily stays down from a knockdown. Raymond then fights Frost, but is defeated by a first-round knockout and hospitalised. Angered, Andre challenges Frost himself. Meagan covertly records one of McQueen's crooked cops mentioning the frame job on Andre, forcing McQueen to agree to the bout. Adopting a defensive strategy, Andre knocks Frost out and becomes the world heavyweight champion. McQueen is subsequently arrested when the framing of Andre is revealed.
Fight Night Champion features over 50 boxers in total through 8 weight divisions (flyweight doesn't have a boxer in its roster unless a boxer has been created), making it the largest roster in the series. New inclusions permit recreations of fights such as Wladimir Klitschko vs. David Haye and Manny Pacquiao vs. Timothy Bradley. There are additional fighters available but downloadable content is not freely available. Some boxers are not licensed for use in the game's bare knuckle mode.
Although endocrine changes are known to modulate the timing of major developmental transitions, the genetic mechanisms underlying these changes remain poorly understood. In insects, two developmental hormones, juvenile hormone (JH) and ecdysteroids, are coordinated with each other to induce developmental changes associated with metamorphosis. However, the regulation underlying the coordination of JH and ecdysteroid synthesis remains elusive. Here, we examined the function of a homolog of the vertebrate POU domain protein, Ventral veins lacking (Vvl)/Drifter, in regulating both of these hormonal pathways in the red flour beetle, Tribolium castaneum (Tenebrionidae). RNA interference-mediated silencing of vvl expression led to both precocious metamorphosis and inhibition of molting in the larva. Ectopic application of a JH analog on vvl knockdown larvae delayed the onset of metamorphosis and led to a prolonged larval stage, indicating that Vvl acts upstream of JH signaling. Accordingly, vvl knockdown also reduced the expression of a JH biosynthesis gene, JH acid methyltransferase 3 (jhamt3). In addition, ecdysone titer and the expression of the ecdysone response gene, hormone receptor 3 (HR3), were reduced in vvl knockdown larvae. The expression of the ecdysone biosynthesis gene phantom (phm) and spook (spo) were reduced in vvl knockdown larvae in the anterior and posterior halves, respectively, indicating that Vvl might influence ecdysone biosynthesis in both the prothoracic gland and additional endocrine sources. Injection of 20-hydroxyecdysone (20E) into vvl knockdown larvae could restore the expression of HR3 although molting was never restored. These findings suggest that Vvl coordinates both JH and ecdysteroid biosynthesis as well as molting behavior to influence molting and the timing of metamorphosis. Thus, in both vertebrates and insects, POU factors modulate the production of major neuroendocrine regulators during sexual maturation.
To determine the function of Vvl in Tribolium, we knocked down the expression of vvl and found that this resulted in precocious metamorphosis. These animals also had lowered expression of the JH response gene, krüppel-homolog 1 (kr-h1), which could be restored with topical application of JH. Knockdown of vvl also resulted in the reduced expression of jhamt3, a key regulator of JH biosynthesis. In addition, vvl knockdown led to an inability to molt and a corresponding reduction in ecdysone levels, and the expressions of ecdysone biosynthesis genes and the ecdysone-response gene, HR3. The expression of HR3 but not the molting defects could be rescued by injection of 20E. Our results suggest that Vvl may act as a nexus between JH and ecdysone biosynthesis.
(A) Expression profile of vvl during the late larval and prepupal stages of Tribolium. Expression profile was determined by qPCR of vvl from whole body sixth and seventh instars and prepupae. Ribosomal protein 49 (rp49) was used as the internal control. For all of the treatments, each sample consisted of RNA pooled from five sixth instars, three seventh instars and three prepupae. Three biological replicates were used per treatment, and each sample was run in triplicates. (B) Expression of vvl in the CNS, epidermis, fat body and gut of day 0 seventh instar larvae. mRNA was isolated from tissues pooled from 20 individuals. (C) Knockdown verification of vvl and Met in early prepupae. Expression profiles for vvl, Met, and rp49 (control) in ampr, vvl, and Met dsRNA-injected animals. Cycle numbers for vvl, Met and rp49 were 34, 35, and 28, respectively. (D) Quantitative real-time PCR data showing the expression of Met (left) and vvl (right) in vvl and Met knockdown prepupae, respectively.
Semi-quantitative RT-PCR was used to verify knockdown of vvl expression in early prepupae after last instar larvae were injected with 0.5 µg of dsRNA. In addition, semi-quantitative RT-PCR and qPCR were used to determine whether knockdown of either vvl or Met leads to changes in Met or expression, respectively. vvl mRNA expression level was lower in animals injected with vvl dsRNA than in the control larvae injected with ampr dsRNA and larvae injected with Met dsRNA (Figures 2C and 2D). In addition, Met expression level was lower in animals injected with Met dsRNA than in those injected with ampr dsRNA or vvl dsRNA (Figure 2C and 2D). These results demonstrate that the vvl and Met expression was effectively reduced and that Met and Vvl do not regulate the mRNA expression of each other. 2b1af7f3a8